Central Dogma of Molecular Biology
Discovery of RDDs by GENCLIS
1) Base substitution: the complementary base is not inserted; instead, the base that precedes or follows the said base replaces it;
2) Gap: the complementary base is omitted; and
3) Insertion: a supernumerary base is added.
As a result of these errors, there exists a non-negligible proportion of mRNA whose message is no longer complementary to the DNA sequence. These mRNAs are translated into proteins, whose amino acid sequences are not determined by the DNA sequence, thus contributing to protein diversity.
Transcriptional Infidelity (TI) is a normal phenomenon that occurs at higher rates in cancer. This phenomenon does not occur randomly, but depends on the context of DNA.
DNA is transcribed into RNA by RNA polymerase II. The RNA is then translated into protein.
During a TI event, RNA polymerase introduces errors in the RNA sequence.
This term refers to the replacement of one base by another. The translation of RNA carrying base substitutions leads to the synthesis of a protein with a modified sequence.
Changes in the amino acid sequences of proteins translated from RNA variants lead to a gain or loss of function.
The consequences of a base deletion for the protein sequence are much more significant than those of a base substitution. A base deletion results in a shift of the reading frame of the protein.
As a result, both the composition and size of the protein sequence are modified. These modifications in the amino acid sequence confer specific physicochemical and immunogenic properties to the protein variants.
Bernard E. Bihain (Chairman of...Read more
Annual Meeting of the American Society of Clinical Oncology
May 29 to June 2, 2015
Chicago, Illinois, USA
SeveridiaTM test results were presented...Read more